Part:BBa_K4806212:Design

POR gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1535
  Illegal PstI site found at 2795
  Illegal PstI site found at 2855
  Illegal PstI site found at 3514
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2720
  Illegal PstI site found at 1535
  Illegal PstI site found at 2795
  Illegal PstI site found at 2855
  Illegal PstI site found at 3514
  Illegal NotI site found at 3160
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1535
  Illegal PstI site found at 2795
  Illegal PstI site found at 2855
  Illegal PstI site found at 3514
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1535
  Illegal PstI site found at 2795
  Illegal PstI site found at 2855
  Illegal PstI site found at 3514
  Illegal NgoMIV site found at 4443
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. PSAD-promotor (A1-B1) (BBa_K4806010)
• 1. CTPPSAD-transit peptide (B2) (BBa_K4806014)
• 2. POR gene (B3-B4) (BBa_K4806003)
• 3. HA-tag (B5) (BBa_K3002017)^*
• 4. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B1-B2 (BBa_K3002302)^*
• 2. Connector B2-B3 (BBa_K3002303)^*
• 3. Connector B4-B5 (BBa_K3002304)^*
• 4. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.